Disc 1: The Book of Jon
- The Big Guy (from Trailer Park Boys)
- Every Move a Picture – Signs of Life
- Harvey Danger – Moral Centralia
- Khaki Snack – New Girlfriend
- Butterfingers – Everytime
- Andrew Bird – Masterfade
- Alkaline Trio – Burn
- Back and Forth (from Me and You and Everyone We Know)
- The Mars Volta – Cygnus…Vismund Cygnus (Edit)
- Kano – Signs in My Life (Edit)
- Kevin Devine – Yr Damned Ol' Dad
- Men, Women and Children – Time for the Future (Bang Bang)
- Trapped in the Closet (from South Park)
- Wolf Parade – This Heart's on Fire
- Black Wire – Very Gun
- Flashlight Arcade – Outside Providence
- All Purpose Voltage Heroes – Punch Out
- Cage – Good Morning (feat. El-P and Matt Sweeney and James McNew of Yo La Tengo)
- The Format – Simple Twist of Fate
- You Know How I Know You're Gay? (from The 40-Year-Old Virgin)
- Sam Champion – You Can't See the Stars in This Town
- Supersystem – Miracle (The Rapture HUSHHUSH Remix)
- Gatsby's American Dream – Just Like Heaven
- Jelly (from It's Always Sunny in Philadelphia)
- Sigur Rós – Sæglópur (Edit)
Disc 2: The Book of Hornblower
- Stewie (from Family Guy)
- …And You Will Know Us by the Trail of Dead – Will You Smile Again? (Instrumental Edit)
- …And You Will Know Us by the Trail of Dead – Worlds Apart
- Skratch Bastid, John Smith, and Pip Skid – Reapin' the Benefits (feat. Sleep)
- Collard Greens (from The Office)
- Mike Doughty – White Lexus
- Weevil – Too Long Sleeping
- Audio Bullys – If You Want My Love
- Bloodhound Gang – Diarrhea Runs in the Family
- Bomb the Music Industry! – Congratulations, John, on Joining Every Time I Die
- Tom McRae – Strangest Land
- Mark Ronson – Just (feat. Alex Greenwald of Phantom Planet)
- Calla – It Dawned on Me
- Mark (from Palindromes)
- Buck 65 – Cries a Girl
- Doomriders – Ride or Die
- Saskrotch – Megaman 2
- She Wants Revenge – Tear You Apart
- Nooooo (from Star Wars: Episode III – Revenge of the Sith)
- Discover America – From the 100th Floor
- Teenage Bottlerocket – Blood Bath at Burger King
- Cursive – Sucker and Dry
- Joe Franklin (from The Aristocrats)
- Death Cab for Cutie – Earth Angel
- Quarashi – Pro
- World Leader Pretend – A Grammarian Stuck in a Medical Drama
- The Murdocks – Easter Moon